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thick fi lter paper  (Bio-Rad)


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    Bio-Rad thick fi lter paper
    Fig. 1 Exosome purifi cation. ( a ) The experimental workfl ow used for fl uorescent Exo isolation based on differential ultracentrifugation. Exo-containing condi- tioned medium from BODIPY fatty acid-labeled cells is processed by differential centrifugation to remove intact cells and cell debris. Resuspended Exo plus con- taminating proteins pellet is fi ltered using a 0.22 <t>μm</t> <t>membrane</t> fi <t>lter</t> before last centrifugation step. The speed and length of each centrifugation are indicated. The fi nal Exo pellet is resuspended in PBS and can be either directly FC counted or ( b ) further purifi ed by running overnight on an 10–40 % OptiPrep density gra- dient. The fl uorescent peak displays a density ranging from 1.06 to 1.15 g/mL typical of Exo [ 10 , 11 ]. ( c ) Western blot analysis of purifi ed exosomes (5 × 10 7 ) probed with antibodies against Exo markers HSP90, Alix, CD63, and Tsg101
    Thick Fi Lter Paper, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1580 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thick fi lter paper/product/Bio-Rad
    Average 96 stars, based on 1580 article reviews
    thick fi lter paper - by Bioz Stars, 2026-02
    96/100 stars

    Images

    1) Product Images from "Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools"

    Article Title: Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools

    Journal: Methods in Molecular Biology

    doi: 10.1007/978-1-4939-3753-0

    Fig. 1 Exosome purifi cation. ( a ) The experimental workfl ow used for fl uorescent Exo isolation based on differential ultracentrifugation. Exo-containing condi- tioned medium from BODIPY fatty acid-labeled cells is processed by differential centrifugation to remove intact cells and cell debris. Resuspended Exo plus con- taminating proteins pellet is fi ltered using a 0.22 μm membrane fi lter before last centrifugation step. The speed and length of each centrifugation are indicated. The fi nal Exo pellet is resuspended in PBS and can be either directly FC counted or ( b ) further purifi ed by running overnight on an 10–40 % OptiPrep density gra- dient. The fl uorescent peak displays a density ranging from 1.06 to 1.15 g/mL typical of Exo [ 10 , 11 ]. ( c ) Western blot analysis of purifi ed exosomes (5 × 10 7 ) probed with antibodies against Exo markers HSP90, Alix, CD63, and Tsg101
    Figure Legend Snippet: Fig. 1 Exosome purifi cation. ( a ) The experimental workfl ow used for fl uorescent Exo isolation based on differential ultracentrifugation. Exo-containing condi- tioned medium from BODIPY fatty acid-labeled cells is processed by differential centrifugation to remove intact cells and cell debris. Resuspended Exo plus con- taminating proteins pellet is fi ltered using a 0.22 μm membrane fi lter before last centrifugation step. The speed and length of each centrifugation are indicated. The fi nal Exo pellet is resuspended in PBS and can be either directly FC counted or ( b ) further purifi ed by running overnight on an 10–40 % OptiPrep density gra- dient. The fl uorescent peak displays a density ranging from 1.06 to 1.15 g/mL typical of Exo [ 10 , 11 ]. ( c ) Western blot analysis of purifi ed exosomes (5 × 10 7 ) probed with antibodies against Exo markers HSP90, Alix, CD63, and Tsg101

    Techniques Used: Isolation, Labeling, Centrifugation, Membrane, Western Blot



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    thick fi lter paper  (Bio-Rad)
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    Bio-Rad thick fi lter paper
    Fig. 1 Exosome purifi cation. ( a ) The experimental workfl ow used for fl uorescent Exo isolation based on differential ultracentrifugation. Exo-containing condi- tioned medium from BODIPY fatty acid-labeled cells is processed by differential centrifugation to remove intact cells and cell debris. Resuspended Exo plus con- taminating proteins pellet is fi ltered using a 0.22 <t>μm</t> <t>membrane</t> fi <t>lter</t> before last centrifugation step. The speed and length of each centrifugation are indicated. The fi nal Exo pellet is resuspended in PBS and can be either directly FC counted or ( b ) further purifi ed by running overnight on an 10–40 % OptiPrep density gra- dient. The fl uorescent peak displays a density ranging from 1.06 to 1.15 g/mL typical of Exo [ 10 , 11 ]. ( c ) Western blot analysis of purifi ed exosomes (5 × 10 7 ) probed with antibodies against Exo markers HSP90, Alix, CD63, and Tsg101
    Thick Fi Lter Paper, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thick fi lter paper/product/Bio-Rad
    Average 96 stars, based on 1 article reviews
    thick fi lter paper - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier
    extra thick fi lter paper  (Bio-Rad)
    94
    Bio-Rad extra thick fi lter paper
    Fig. 1. Biochemical analysis of naturally processed peptides. Cells are homogenized in acid and fi ltered through a 3–30 kDa molecular fi <t>lter.</t> The fi ltrate containing the complex peptide mixture is then fractionated by reverse phase HPLC. The fractions, collected in 96-well plates, are dried and resuspended in buffer. Appropriate APC and T cells are added to the wells and after overnight culture, the T cell response is measured by the conversion of the lacZ substrate to a colored product. In the schematic shown, the naturally processed peptide in extracts of OVA expressing cells co-elutes with the synthetic SIINFEKL (SL8) peptide. Mock injections of buffer alone are carried out prior to each run to ensure that the activity in the test fractions is not due to spurious cross-contamination between samples.
    Extra Thick Fi Lter Paper, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/extra thick fi lter paper/product/Bio-Rad
    Average 94 stars, based on 1 article reviews
    extra thick fi lter paper - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 1 Exosome purifi cation. ( a ) The experimental workfl ow used for fl uorescent Exo isolation based on differential ultracentrifugation. Exo-containing condi- tioned medium from BODIPY fatty acid-labeled cells is processed by differential centrifugation to remove intact cells and cell debris. Resuspended Exo plus con- taminating proteins pellet is fi ltered using a 0.22 μm membrane fi lter before last centrifugation step. The speed and length of each centrifugation are indicated. The fi nal Exo pellet is resuspended in PBS and can be either directly FC counted or ( b ) further purifi ed by running overnight on an 10–40 % OptiPrep density gra- dient. The fl uorescent peak displays a density ranging from 1.06 to 1.15 g/mL typical of Exo [ 10 , 11 ]. ( c ) Western blot analysis of purifi ed exosomes (5 × 10 7 ) probed with antibodies against Exo markers HSP90, Alix, CD63, and Tsg101

    Journal: Methods in Molecular Biology

    Article Title: Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools

    doi: 10.1007/978-1-4939-3753-0

    Figure Lengend Snippet: Fig. 1 Exosome purifi cation. ( a ) The experimental workfl ow used for fl uorescent Exo isolation based on differential ultracentrifugation. Exo-containing condi- tioned medium from BODIPY fatty acid-labeled cells is processed by differential centrifugation to remove intact cells and cell debris. Resuspended Exo plus con- taminating proteins pellet is fi ltered using a 0.22 μm membrane fi lter before last centrifugation step. The speed and length of each centrifugation are indicated. The fi nal Exo pellet is resuspended in PBS and can be either directly FC counted or ( b ) further purifi ed by running overnight on an 10–40 % OptiPrep density gra- dient. The fl uorescent peak displays a density ranging from 1.06 to 1.15 g/mL typical of Exo [ 10 , 11 ]. ( c ) Western blot analysis of purifi ed exosomes (5 × 10 7 ) probed with antibodies against Exo markers HSP90, Alix, CD63, and Tsg101

    Article Snippet: Nitrocellulose membrane (0.45 μm, Bio-Rad Laboratories) and thick fi lter paper (Bio-Rad Laboratories).

    Techniques: Isolation, Labeling, Centrifugation, Membrane, Western Blot

    Fig. 1. Biochemical analysis of naturally processed peptides. Cells are homogenized in acid and fi ltered through a 3–30 kDa molecular fi lter. The fi ltrate containing the complex peptide mixture is then fractionated by reverse phase HPLC. The fractions, collected in 96-well plates, are dried and resuspended in buffer. Appropriate APC and T cells are added to the wells and after overnight culture, the T cell response is measured by the conversion of the lacZ substrate to a colored product. In the schematic shown, the naturally processed peptide in extracts of OVA expressing cells co-elutes with the synthetic SIINFEKL (SL8) peptide. Mock injections of buffer alone are carried out prior to each run to ensure that the activity in the test fractions is not due to spurious cross-contamination between samples.

    Journal: Methods in Molecular Biology

    Article Title: Antigen Processing

    doi: 10.1007/978-1-62703-218-6

    Figure Lengend Snippet: Fig. 1. Biochemical analysis of naturally processed peptides. Cells are homogenized in acid and fi ltered through a 3–30 kDa molecular fi lter. The fi ltrate containing the complex peptide mixture is then fractionated by reverse phase HPLC. The fractions, collected in 96-well plates, are dried and resuspended in buffer. Appropriate APC and T cells are added to the wells and after overnight culture, the T cell response is measured by the conversion of the lacZ substrate to a colored product. In the schematic shown, the naturally processed peptide in extracts of OVA expressing cells co-elutes with the synthetic SIINFEKL (SL8) peptide. Mock injections of buffer alone are carried out prior to each run to ensure that the activity in the test fractions is not due to spurious cross-contamination between samples.

    Article Snippet: Extra-thick fi lter paper (Bio-Rad).

    Techniques: Expressing, Activity Assay